Eosinophil Ribonucleases and Their Cutaneous Lesion-Forming Activity

Cutaneous Lesion-Forming Activity Eosinophil Ribonucleases and Their

Eosinophil ribonucleases and their cutaneous lesion-forming activity.

Eosinophil granule proteins are deposited in cutaneous lesions in many human diseases, but how these proteins contribute to pathophysiology is obscure. We injected eosinophil cationic protein (ECP or RNase 3), eosinophil-derived neurotoxin (EDN or RNase 2), eosinophil peroxidase (EPO), and major basic protein-1 (MBP1) intradermally into guinea pig and rabbit skin. ECP and EDN each induced disti...

Eosinophil and neutrophil colony-forming cells in culture.

Clusters of cells obtained from a short-term culture of human bone marrow cells in methylcellulose were transplanted in cell-free plates containing conditioned medium. Of 2559 transplants, 439 gave small clusters, 223 gave large clusters, and 70 gave colonies. A better cloning efficiency was achieved from 4-day-old clusters, from aggregates containing 3 cells or more, and from large cells rathe...

An Overview on Ribonucleases and their Therapeutic Effects

The maturation and degradation of ribonucleic acid (RNA) into smaller nucleotides are carried out by a wide variety of cellular ribonucleases (RNases) which are also responsible for regulating the functional expression of several fundamental genes in living systems. They exist in eukaryotes to prokaryotes as well as in viruses. Structure, functions and catalytic mechanism of RNases have been we...

Effective and lesion-free cutaneous influenza vaccination.

The current study details efficient lesion-free cutaneous vaccination via vaccine delivery into an array of micropores in the skin, instead of bolus injection at a single site. Such delivery effectively segregated vaccine-induced inflammation, resulting in rapid resolution of the inflammation, provided that distances between any two micropores were sufficient. When the inoculation site was trea...